Technology · Purification
23-Step Purification
Process
Biologically-driven enzyme-based purification without chemical solvents. All 23 stages from raw material intake to final shipment are managed to ISO 9001 standards.
STEP 1-3
Raw Material Preprocessing
STEP 4-9
Enzyme Treatment
STEP 10-17
Purification & Classification
STEP 18-23
Final Validation
Phase 1 · STEP 1-3
Raw Material Preprocessing
Raw Material Intake
Freshwater sponges cultivated naturally and harvested in pristine waters (SPICULE raw material)
Origin Verification
Certificate of origin review, CoA verification, and origin traceability confirmation
Comprehensive Microbial Testing
TPC, Yeast & Mold limit testing
Phase 2 · STEP 4-9
Enzyme Treatment
Protease Enzyme Solution Addition
Enzyme culture solution is added in a controlled environment
Enzyme Treatment Initiation
Enzyme treatment starts under real-time monitoring of pH, temperature and dissolved oxygen
72-Hour Enzyme Treatment Stabilization
72+ hours of continuous enzyme treatment under 24/7 automated SCADA monitoring
Enzyme Treatment Completion Assessment
Completion is judged against endpoint indicators (pH, turbidity, microbial count)
Enzyme-Treated Material Separation
Bioactive components are separated and recovered under non-detectable (ND) residual solvent conditions
Multi-Mesh Filtering
Physical impurities are removed with stepwise mesh filters
Phase 3 · STEP 10-17
Purification & Classification
Centrifugal Separation
Solids are separated from the supernatant with a centrifuge
Multiple Rinse Cycles
Residual organic matter is removed by repeated high-pressure rinsing with purified water
Heat Treatment
Heating completely eliminates residual microorganisms such as enzymes
Dehydration
Moisture is removed by heated dehydration
Drying
Dried for 24 hours at 80°C
Multi-Mesh Classification
Particle size is classified with stepwise 100–300 mesh filters
Particle Size Verification
Particle size and shape are verified batch by batch with optical (microscope) inspection
Surface Treatment
Optional post-treatments such as biopolymer coating, functional ingredient impregnation and encapsulation
Phase 4 · STEP 18-23
Final Validation
Heavy Metal Testing
Full heavy metal testing for As, Pb, Hg and Cd
Final Microbial Limit Testing
Microbial limit testing of the final product (TPC, E. coli, S. aureus)
Sensory & Foreign Matter Inspection
Sensory inspection of appearance, color and odor, plus optical and UV microscope foreign matter inspection
Primary Packing & Gamma Irradiation / Ozone Treatment
Gamma irradiation and ozone treatment after primary packaging
Final Quality Judgment
Comprehensive judgment of all test results and shipment approval
Secondary Packaging & CoA Release
Secondary packaging before shipment, with a lot-specific CoA issued at release
Result
Purification Before & After Comparison
Raw Material State
Mixed with impurities
Final Product
High-purity SPICULE
99%
Final Purity
ND
Residual Solvent
CV<5%
Particle Size Uniformity
24/7
SCADA Monitoring
Purification Technology Inquiry
We welcome OEM/ODM raw material supply and technical partnership collaborations.